KMID : 1134820080370040405
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Journal of the Korean Society of Food Science and Nutrition 2008 Volume.37 No. 4 p.405 ~ p.409
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Antioxidant Activity and Inhibition Activity against ¥á-Amylase and ¥á-Glucosidase of Viola mandshurica Extracts
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Lee Bo-Bae
Park Soon-Rye Han Chang-Suk Han Dong-Youl Park Eun-Ju Park Hae-Ryong Lee Seung-Cheol
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Abstract
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This study was performed to investigate the physiological activities of Viola mandshurica. Antioxidant
activity was evaluated by measuring total phenolic contents, reducing power, 1,1-diphenyl-2-picrylhydrazyl
(DPPH) radical scavenging activity, 2,2¡¯-azino-di-2-ethyl-benzthiazoline sulphonate (ABTS) radical
scavenging activity while anti-diabetic activity was measured by inhibition activities on ¥á-amylase and ¥á-
glucosidase. V. mandshurica extracts were prepared by extracting with four different solvents (methanol,
ethanol, acetone, and water). The methanol extract showed the highest total phenol content (34.49 mg/g gallic
acid equivalents) among the extracts. The water extract showed the highest reducing power (0.454) at the
concentration of 1,000 ¥ìg/mL. The acetone extract showed the most potent radical scavenging activity. DPPH
and ABTS radical scavenging activity of the acetone extract at the concentration of 1,000 ¥ìg/mL were 21.13%
and 43.53%, respectively. The inhibitory activity of acetone extracts against ¥á-amylase and ¥á-glucosidase
showed more than 100% at the concentration of 1,000 ¥ìg/mL. The results indicate that V. mandshurica might
have potential antioxidant and anti-diabetic activities.
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KEYWORD
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Viola mandshurica, antioxidan, ¥á-amylase, ¥á-glucosidase
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